4N56
Binary complex structure of Klenow fragment of Taq DNA polymerase I707L mutant (Cs3C KlenTaq) with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.040, 110.040, 91.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.650 - 2.200 |
| R-factor | 0.22532 |
| Rwork | 0.223 |
| R-free | 0.27323 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ktq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.715 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.650 | 2.257 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 32282 | |
| <I/σ(I)> | 11.97 | 2.75 |
| Completeness [%] | 98.5 | 98.6 |
| Redundancy | 3.6 | 3.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M HEPES, pH 7.5, 20 mM manganese chloride, 0.1 M sodium acetate, 10% w/v PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
