4MVJ

2.85 Angstrom Resolution Crystal Structure of Glyceraldehyde 3-phosphate Dehydrogenase A (gapA) from Escherichia coli Modified by Acetyl Phosphate.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, K, N (A, B, C, K, N)	Glyceraldehyde-3-phosphate dehydrogenase A	polymer	355	38362.4	5	UniProt (C9QTS9)	Escherichia coli	Glyceraldehyde-3-phosphate dehydrogenase, type I
2	D, L (D, L)	Glyceraldehyde-3-phosphate dehydrogenase A	polymer	355	38321.3	2	UniProt (C9QTS9)	Escherichia coli	Glyceraldehyde-3-phosphate dehydrogenase, type I
3	E (E)	Glyceraldehyde-3-phosphate Dehydrogenase A	polymer	355	38403.4	1	UniProt (C9QTS9)	Escherichia coli
4	F, J, O (F, J, O)	Glyceraldehyde-3-phosphate Dehydrogenase A	polymer	355	38403.4	3	UniProt (C9QTS9)	Escherichia coli
5	G (G)	Glyceraldehyde-3-phosphate Dehydrogenase A	polymer	355	38404.4	1	UniProt (C9QTS9)	Escherichia coli
6	H (H)	Glyceraldehyde-3-phosphate Dehydrogenase A	polymer	355	38445.4	1	UniProt (C9QTS9)	Escherichia coli
7	I (I)	Glyceraldehyde-3-phosphate Dehydrogenase A	polymer	355	38444.4	1	UniProt (C9QTS9)	Escherichia coli
8	M (M)	Glyceraldehyde-3-phosphate Dehydrogenase A	polymer	355	38362.4	1	UniProt (C9QTS9)	Escherichia coli
9	P (P)	Glyceraldehyde-3-phosphate Dehydrogenase A	polymer	355	38445.4	1	UniProt (C9QTS9)	Escherichia coli
10	AA, AB, IC, KA, KB... (B, E, K, C, G...)	SODIUM ION	non-polymer		23.0	12	Chemie (NA)
11	AD, BA, BB, BC, CA... (O, B, E, J, P...)	CHLORIDE ION	non-polymer		35.5	49	Chemie (CL)
12	AC, DC, EC, ED, FD... (I, J, P, E, B...)	PHOSPHATE ION	non-polymer		95.0	15	Chemie (PO4)
13	GC, IA, JB, PB, VC... (J, B, F, G, M...)	PYROPHOSPHATE 2-	non-polymer		176.0	6	Chemie (POP)
14	BD, HC, JA, QA, WB... (P, K, C, D, I...)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	7	Chemie (NAD)
15	TB (H)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
16	UB (H)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
17	VB (H)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
18	FC (J)	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
19	UC (M)	ACETYLPHOSPHATE	non-polymer		140.0	1	Chemie (UVW)
20	GD, HD, ID, JD, KD... (A, B, C, D, E...)	water	water		18.0	443	Chemie (HOH)

Sequence modifications

A, B, C, K, N: 1 - 331 (UniProt: C9QTS9)

PDB	External Database	Details
Met -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

D, L: 1 - 331 (UniProt: C9QTS9)

PDB	External Database	Details
Met -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

E: 1 - 331 (UniProt: C9QTS9)

PDB	External Database	Details
Met -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

F, J, O: 1 - 331 (UniProt: C9QTS9)

PDB	External Database	Details
Met -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

G: 1 - 331 (UniProt: C9QTS9)

PDB	External Database	Details
Met -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

H: 1 - 331 (UniProt: C9QTS9)

PDB	External Database	Details
Met -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

I: 1 - 331 (UniProt: C9QTS9)

PDB	External Database	Details
Met -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

M: 1 - 331 (UniProt: C9QTS9)

PDB	External Database	Details
Met -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

P: 1 - 331 (UniProt: C9QTS9)

PDB	External Database	Details
Met -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	614170.3
Non-Polymers*	Number of molecules	94
	Total formula weight	9787.0
All*	Total formula weight	623957.2

*Water molecules are not included.