4MVJ
2.85 Angstrom Resolution Crystal Structure of Glyceraldehyde 3-phosphate Dehydrogenase A (gapA) from Escherichia coli Modified by Acetyl Phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 145.879, 69.688, 271.923 |
| Unit cell angles | 90.00, 98.80, 90.00 |
Refinement procedure
| Resolution | 29.910 - 2.850 |
| R-factor | 0.18774 |
| Rwork | 0.186 |
| R-free | 0.22350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7c |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.904 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.086 | 0.637 |
| Number of reflections | 127301 | |
| <I/σ(I)> | 15.5 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | Protein - 7.3mg/mL, 0.15M Sodium chloride, 0.01M Tris-HCl pH 8.3, Screen - PACT (G2), 0.2 M Sodium bromide, 0.1 M Bis Tris propane pH 7.5, 20 % (w/v) PEG 3350, 10mM ACP., VAPOR DIFFUSION, SITTING DROP, temperature 295K |
