4MRT
Structure of the Phosphopantetheine Transferase Sfp in Complex with Coenzyme A and a Peptidyl Carrier Protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | Tyrocidine synthase 3 | polymer | 90 | 9972.4 | 1 | UniProt (O30409) Pfam (PF00550) | Brevibacillus parabrevis | Tyrocidine synthase III, ATP-dependent asparagine adenylase, AsnA, Asparagine activase, ATP-dependent glutamine adenylase, GlnA, Glutamine activase, ATP-dependent tyrosine adenylase, TyrA, Tyrosine activase, ATP-dependent valine adenylase, ValA, Valine activase, ATP-dependent ornithine adenylase, OrnA, Ornithine activase, ATP-dependent leucine adenylase, LeuA, Leucine activase |
| 2 | B (A) | 4'-phosphopantetheinyl transferase sfp | polymer | 232 | 27245.8 | 1 | UniProt (P39135) Pfam (PF22624) Pfam (PF01648) | Bacillus subtilis subsp. subtilis | Surfactin synthase-activating enzyme |
| 3 | C, F (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 4 | D (A) | COENZYME A | non-polymer | 767.5 | 1 | Chemie (COA) | |||
| 5 | E (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | G (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 7 | H, I (C, A) | water | water | 18.0 | 172 | Chemie (HOH) |
Sequence modifications
C: 8 - 83 (UniProt: O30409)
A: 1 - 224 (UniProt: P39135)
| PDB | External Database | Details |
|---|---|---|
| Pro 2 | - | expression tag |
| Val 3 | - | expression tag |
| Thr 4 | - | expression tag |
| Glu 5 | - | expression tag |
| Ala 6 | - | expression tag |
| Gln 7 | - | expression tag |
| Ala 45 | Ser 3075 | engineered mutation |
| Arg 84 | - | cloning artifact |
| Ser 85 | - | cloning artifact |
| His 86 | - | expression tag |
| His 87 | - | expression tag |
| His 88 | - | expression tag |
| His 89 | - | expression tag |
| His 90 | - | expression tag |
| His 91 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Thr 22 | Ser 22 | variant |
| Gly 97 | Cys 97 | variant |
| Arg 225 | - | cloning artifact |
| Ser 226 | - | cloning artifact |
| His 227 | - | expression tag |
| His 228 | - | expression tag |
| His 229 | - | expression tag |
| His 230 | - | expression tag |
| His 231 | - | expression tag |
| His 232 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 37218.2 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1004.3 | |
| All* | Total formula weight | 38222.5 |
