4MRT
Structure of the Phosphopantetheine Transferase Sfp in Complex with Coenzyme A and a Peptidyl Carrier Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.660, 39.090, 53.310 |
| Unit cell angles | 90.00, 107.03, 90.00 |
Refinement procedure
| Resolution | 37.910 - 2.000 |
| R-factor | 0.1906 |
| Rwork | 0.188 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.502 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.910 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 21594 |
| <I/σ(I)> | 6.7 |
| Completeness [%] | 99.5 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.16 M magnesium acetate, 0.08 M sodium cacodylate, 16% (w/v) PEG 8000, 20% (v/v) glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
