4MGJ

Crystal structure of cytochrome P450 2B4 F429H in complex with 4-CPI

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cytochrome P450 2B4	polymer	476	54170.1	1	UniProt (P00178) Pfam (PF00067) In PDB	Oryctolagus cuniculus (European rabbit Japanese white rabbit domestic rabbit rabbits)	CYPIIB4, Cytochrome P450 isozyme 2, Cytochrome P450 LM2, Cytochrome P450 type B0, Cytochrome P450 type B1
2	A	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
3	A	4-(4-CHLOROPHENYL)IMIDAZOLE	non-polymer		178.6	1	Chemie (CPZ)
4		water	water		18.0	106	Chemie (HOH)

Sequence modifications

A: 30 - 491 (UniProt: P00178)

PDB	External Database	Details
Met 20	-	initiating methionine
Ala 21	-	expression tag
Lys 22	-	expression tag
Lys 23	-	expression tag
Thr 24	-	expression tag
Ser 25	-	expression tag
Ser 26	-	expression tag
Lys 27	-	expression tag
Gly 28	-	expression tag
Lys 29	-	expression tag
Tyr 226	His 226	engineered mutation
His 429	Phe 429	engineered mutation
His 492	-	expression tag
His 493	-	expression tag
His 494	-	expression tag
His 495	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	54170.1
Non-Polymers*	Number of molecules	2
	Total formula weight	795.1
All*	Total formula weight	54965.2

*Water molecules are not included.