4M0W

Crystal Structure of SARS-CoV papain-like protease C112S mutant in complex with ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Replicase polyprotein 1a	polymer	327	36858.7	1	UniProt (P0C6U8) Pfam (PF08715) In PDB	SARS coronavirus (SARS-CoV)
2	B	Ubiquitin	polymer	76	8576.8	1	UniProt (P62992) Pfam (PF00240) In PDB	Bos taurus (bovine)
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	A	2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID	non-polymer		207.3	2	Chemie (NHE)
6	A, B	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
7		water	water		18.0	313	Chemie (HOH)

Sequence modifications

A: 2 - 319 (UniProt: P0C6U8)

PDB	External Database	Details
Met 1	-	expression tag
Ser 112	Cys 1651	engineered mutation
Leu 320	-	expression tag
Glu 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45435.5
Non-Polymers*	Number of molecules	7
	Total formula weight	779.3
All*	Total formula weight	46214.8

*Water molecules are not included.