4LK0
Crystal Structure Analysis of the E.coli holoenzyme/T7 Gp2 complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, G, H | DNA-directed RNA polymerase subunit alpha | polymer | 239 | 26459.1 | 4 | UniProt (C9QXI7) In PDB | Escherichia coli | RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha |
2 | C, I | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 2 | UniProt (C9QV90) In PDB | Escherichia coli | RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta |
3 | D, J | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 2 | UniProt (C5A0S8) In PDB | Escherichia coli | |
4 | E, K | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 2 | UniProt (C9QUL2) In PDB | Escherichia coli | RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega |
5 | F, L | RNA polymerase sigma factor RpoD | polymer | 522 | 60315.3 | 2 | UniProt (P00579) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Escherichia coli | Sigma-70 |
6 | M, N | Bacterial RNA polymerase inhibitor | polymer | 64 | 7181.0 | 2 | UniProt (P03704) Pfam (PF16857) In PDB | Enterobacteria phage T7 | |
7 | D, J | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
8 | D, J | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
A, B, G, H: 1 - 234 (UniProt: C9QXI7)
PDB | External Database | Details |
---|---|---|
Glu 235 | - | expression tag |
Val 236 | - | expression tag |
Leu 237 | - | expression tag |
Phe 238 | - | expression tag |
Gln 239 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 14 |
Total formula weight | 873703.4 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 310.2 | |
All* | Total formula weight | 874013.6 |