4LK0

Crystal Structure Analysis of the E.coli holoenzyme/T7 Gp2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, G, H	DNA-directed RNA polymerase subunit alpha	polymer	239	26459.1	4	UniProt (C9QXI7) In PDB	Escherichia coli	RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha
2	C, I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	2	UniProt (C9QV90) In PDB	Escherichia coli	RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta
3	D, J	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	2	UniProt (C5A0S8) In PDB	Escherichia coli
4	E, K	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	2	UniProt (C9QUL2) In PDB	Escherichia coli	RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega
5	F, L	RNA polymerase sigma factor RpoD	polymer	522	60315.3	2	UniProt (P00579) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Escherichia coli	Sigma-70
6	M, N	Bacterial RNA polymerase inhibitor	polymer	64	7181.0	2	UniProt (P03704) Pfam (PF16857) In PDB	Enterobacteria phage T7
7	D, J	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	D, J	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A, B, G, H: 1 - 234 (UniProt: C9QXI7)

PDB	External Database	Details
Glu 235	-	expression tag
Val 236	-	expression tag
Leu 237	-	expression tag
Phe 238	-	expression tag
Gln 239	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	873703.4
Non-Polymers*	Number of molecules	6
	Total formula weight	310.2
All*	Total formula weight	874013.6

*Water molecules are not included.