4L8V
Crystal Structure of A12K/D35S mutant myo-inositol dehydrogenase from Bacillus subtilis with bound cofactor NADP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase | polymer | 337 | 37646.8 | 4 | UniProt (P26935) Pfam (PF01408) Pfam (PF02894) | Bacillus subtilis subsp. subtilis | Myo-inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase, MI 2-dehydrogenase/DCI 3-dehydrogenase |
| 2 | E, H, I, L (A, B, C, D) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 4 | Chemie (NAP) | |||
| 3 | F, G, J, K, M (A, C, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
| 4 | N, O, P, Q (A, B, C, D) | water | water | 18.0 | 218 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 337 (UniProt: P26935)
| PDB | External Database | Details |
|---|---|---|
| Lys 12 | Ala 12 | engineered mutation |
| Ser 35 | Asp 35 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 150587.3 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 3284.0 | |
| All* | Total formula weight | 153871.2 |
