4KVG

Crystal structure of RIAM RA-PH domains in complex with GTP bound Rap1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ras-related protein Rap-1A	polymer	168	19236.9	2	UniProt (P62834) Pfam (PF00071)	Homo sapiens (human)	C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1
2	B, D (B, D)	Amyloid beta A4 precursor protein-binding family B member 1-interacting protein	polymer	260	30902.5	2	UniProt (Q8R5A3) Pfam (PF00788) Pfam (PF21989) Pfam (PF00169)	Mus musculus (mouse)	APBB1-interacting protein 1, Proline-rich EVH1 ligand 1, PREL-1, Proline-rich protein 48
3	E, H (A, C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
4	F, I (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	G, J (B, D)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	K, L, M, N (A, B, C, D)	water	water		18.0	725	Chemie (HOH)

Sequence modifications

A, C: 1 - 167 (UniProt: P62834)

PDB	External Database	Details
His 0	-	expression tag
Val 12	Gly 12	engineered mutation
Glu 63	Gln 63	engineered mutation

B, D: 179 - 437 (UniProt: Q8R5A3)

PDB	External Database	Details
Met 178	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	100278.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1219.1
All*	Total formula weight	101497.8

*Water molecules are not included.