4KVG

Crystal structure of RIAM RA-PH domains in complex with GTP bound Rap1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ras-related protein Rap-1A	polymer	168	19236.9	2	UniProt (P62834) Pfam (PF00071)	Homo sapiens (human)	C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1
2	B, D (B, D)	Amyloid beta A4 precursor protein-binding family B member 1-interacting protein	polymer	260	30902.5	2	UniProt (Q8R5A3) Pfam (PF00788) Pfam (PF00169)	Mus musculus (mouse)	APBB1-interacting protein 1, Proline-rich EVH1 ligand 1, PREL-1, Proline-rich protein 48
3	E, H (A, C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
4	F, I (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
5	G, J (B, D)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	K, L, M, N (A, B, C, D)	water	water		18.0	725	Chemie (HOH)

Sequence modifications

A, C: 1 - 167 (UniProt: P62834)

PDB	External Database	Details
His 0	-	expression tag
Val 12	Gly 12	engineered mutation
Glu 63	Gln 63	engineered mutation

B, D: 179 - 437 (UniProt: Q8R5A3)

PDB	External Database	Details
Met 178	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	100278.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1219.1
All*	Total formula weight	101497.8

*Water molecules are not included.