4KVG
Crystal structure of RIAM RA-PH domains in complex with GTP bound Rap1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.467, 85.848, 160.852 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.820 - 1.650 |
R-factor | 0.19821 |
Rwork | 0.197 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.070 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 130322 | |
<I/σ(I)> | 2.1 | |
Completeness [%] | 99.4 | 93.8 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M ammonium sulfate, 0.1M Tris, 15% PEG 1500, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |