4KRM

Nanobody/VHH domain 7D12 in complex with domain III of the extracellular region of EGFR, pH 3.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I...	Epidermal growth factor receptor	polymer	214	23786.1	6	UniProt (P00533) Pfam (PF01030) In PDB	Homo sapiens (human)	Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
2	B, D, F, H, J...	Nanobody/VHH domain 7D12	polymer	133	14575.9	6		Lama glama (llama)
3	M, N, P, Q, R...	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	6	In PDB GlyTouCan (G81315DD)
4	O	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	I, K, A, C, E...	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	11	Chemie (NAG)
6		water	water		18.0	92	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K: 311 - 514 (UniProt: P00533)

PDB	External Database	Details
Leu 307	-	expression tag
Glu 308	-	expression tag
Glu 309	-	expression tag
Lys 310	-	expression tag
His 515	-	expression tag
His 516	-	expression tag
His 517	-	expression tag
His 518	-	expression tag
His 519	-	expression tag
His 520	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	230172.0
Branched	Number of molecules	7
	Total formula weight	4916.5
Non-Polymers*	Number of molecules	11
	Total formula weight	2433.3
All*	Total formula weight	237521.8

*Water molecules are not included.