4KRM
Nanobody/VHH domain 7D12 in complex with domain III of the extracellular region of EGFR, pH 3.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.702, 147.245, 254.799 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.514 - 2.655 |
R-factor | 0.2099 |
Rwork | 0.208 |
R-free | 0.24430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3B2U AND 4KRN |
RMSD bond length | 0.005 |
RMSD bond angle | 0.855 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.700 |
High resolution limit [Å] | 2.650 | 7.190 | 2.650 |
Rmerge | 0.108 | 0.044 | 0.614 |
Number of reflections | 83116 | ||
<I/σ(I)> | 8.5 | ||
Completeness [%] | 96.6 | 99.8 | 75.4 |
Redundancy | 6.8 | 7 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 22.5% PEG3350, 0.1 M sodium citrate, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |