4KAX

Crystal structure of the Grp1 PH domain in complex with Arf6-GTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	ADP-ribosylation factor 6	polymer	169	19530.3	1	UniProt (P62330) Pfam (PF00025) In PDB	Homo sapiens (human)
2	B	Cytohesin-3	polymer	161	19038.6	1	UniProt (O43739) Pfam (PF00169) In PDB	Homo sapiens (human)	ARF nucleotide-binding site opener 3, Protein ARNO3, General receptor of phosphoinositides 1, Grp1, PH, SEC7 and coiled-coil domain-containing protein 3
3	A	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	A	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
6	A, B	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
7	A	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
8	B	INOSITOL-(1,3,4,5)-TETRAKISPHOSPHATE	non-polymer		500.1	1	Chemie (4IP)
9		water	water		18.0	426	Chemie (HOH)

Sequence modifications

A: 14 - 173 (UniProt: P62330)

PDB	External Database	Details
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
Gly 12	-	expression tag
Ser 13	-	expression tag
Leu 67	Gln 67	engineered mutation
Asn 174	-	expression tag

B: 247 - 399 (UniProt: O43739)

PDB	External Database	Details
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
Gly 245	-	expression tag
Ser 246	-	expression tag
-	Gly 275	deletion

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	38568.9
Non-Polymers*	Number of molecules	9
	Total formula weight	1647.2
All*	Total formula weight	40216.1

*Water molecules are not included.