4JSC

The 2.5A crystal structure of humanized Xenopus MDM2 with RO5316533 - a pyrrolidine MDM2 inhibitor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	E3 ubiquitin-protein ligase Mdm2	polymer	86	9962.6	2	UniProt (P56273) Pfam (PF02201)	Xenopus laevis (clawed frog common platanna platanna)	Double minute 2 protein, Xdm2, p53-binding protein Mdm2
2	C, D (A, B)	(3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-N-[(3S)-3,4-dihydroxybutyl]-5-(2,2-dimethylpropyl)-D-prolinamide	non-polymer		554.5	2	Chemie (1OY)
3	E, F (A, B)	water	water		18.0	52	Chemie (HOH)

Sequence modifications

A, B: 21 - 105 (UniProt: P56273)

PDB	External Database	Details
Met 20	-	initiating methionine
Leu 50	Ile 50	engineered mutation
His 92	Pro 92	engineered mutation
Ile 95	Leu 95	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	19925.2
Non-Polymers*	Number of molecules	2
	Total formula weight	1108.9
All*	Total formula weight	21034.1

*Water molecules are not included.