4JAS
Structural basis of a rationally rewired protein-protein interface (HK853mutant A268V, A271G, T275M, V294T and D297E and RR468mutant V13P, L14I, I17M and N21V)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Histidine kinase | polymer | 258 | 29438.4 | 1 | UniProt (Q9WZV7) Pfam (PF00512) Pfam (PF02518) | Thermotoga maritima | Sensor histidine kinase |
| 2 | B (B) | Response regulator | polymer | 122 | 13914.3 | 1 | UniProt (Q9WYT9) Pfam (PF00072) | Thermotoga maritima | RR468, Response regulator receiver protein |
| 3 | C (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 4 | D, E (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | F, G (A, B) | water | water | 18.0 | 13 | Chemie (HOH) |
Sequence modifications
A: 232 - 489 (UniProt: Q9WZV7)
B: 1 - 122 (UniProt: Q9WYT9)
| PDB | External Database | Details |
|---|---|---|
| Val 268 | Ala 268 | engineered mutation |
| Gly 271 | Ala 271 | engineered mutation |
| Met 275 | Thr 275 | engineered mutation |
| Thr 294 | Val 294 | engineered mutation |
| Glu 297 | Asp 297 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Pro 13 | Val 13 | engineered mutation |
| Ile 14 | Leu 14 | engineered mutation |
| Met 17 | Ile 17 | engineered mutation |
| Val 21 | Asn 21 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 43352.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 475.8 | |
| All* | Total formula weight | 43828.5 |
