4IW3

Crystal structure of a Pseudomonas putida prolyl-4-hydroxylase (P4H) in complex with elongation factor Tu (EF-Tu)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, J)	Putative uncharacterized protein	polymer	229	25640.7	2	UniProt (Q88CM1) Pfam (PF13640)	Pseudomonas putida	Prolyl-4-hydroxylase
2	B, C (B, K)	Elongation factor Tu-A	polymer	433	47378.9	2	UniProt (Q88QP8) Pfam (PF00009) Pfam (PF03144) Pfam (PF03143)	Pseudomonas putida	EF-Tu-A
3	E, K (A, J)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	F, L (A, J)	N-OXALYLGLYCINE	non-polymer		147.1	2	Chemie (OGA)
5	G, J (B, K)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
6	H, I (B, K)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	M, N, O, P (A, B, K, J)	water	water		18.0	166	Chemie (HOH)

Sequence modifications

A, J: 2 - 207 (UniProt: Q88CM1)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Leu -8	-	expression tag
Val -7	-	expression tag
Pro -6	-	expression tag
Arg -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag

B, K: 1 - 397 (UniProt: Q88QP8)

PDB	External Database	Details
Met -35	-	expression tag
Gly -34	-	expression tag
Ser -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Leu -22	-	expression tag
Val -21	-	expression tag
Pro -20	-	expression tag
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Met -15	-	expression tag
Ala -14	-	expression tag
Ser -13	-	expression tag
Met -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gln -8	-	expression tag
Gln -7	-	expression tag
Met -6	-	expression tag
Gly -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	146039.2
Non-Polymers*	Number of molecules	8
	Total formula weight	1339.1
All*	Total formula weight	147378.3

*Water molecules are not included.