4IW3
Crystal structure of a Pseudomonas putida prolyl-4-hydroxylase (P4H) in complex with elongation factor Tu (EF-Tu)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979500 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 200.704, 200.704, 74.833 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.558 | 29.558 | 2.860 |
High resolution limit [Å] | 2.697 | 7.830 | 2.700 |
Rmerge | 0.086 | 0.013 | 0.013 |
Rmeas | 0.031 | 1.437 | |
Number of reflections | 47788 | 3729 | 14842 |
<I/σ(I)> | 13.37 | 39.98 | 1.7 |
Completeness [%] | 99.8 | 98 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.8mM MnCl2, 0.9mM OGA, 1mM GDP, 0.1M MgCl2, 22% polyacrylate 5100, 0.1M Hepes pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |