4IRU

Crystal Structure of lepB GAP core in a transition state mimetic complex with Rab1A and ALF3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	LepB	polymer	299	33916.3	3	UniProt (Q5ZSM7) Pfam (PF18172) Pfam (PF18227) In PDB	Legionella pneumophila
2	B, D, F	Ras-related protein Rab-1A	polymer	184	21175.2	3	UniProt (P62820) Pfam (PF00071) In PDB	Homo sapiens (human)	YPT1-related protein
3	C, A, D, B	ACETATE ION	non-polymer		59.0	12	Chemie (ACT)
4	C, A, B	GLYCEROL	non-polymer		92.1	8	Chemie (GOL)
5	C, A, B	POTASSIUM ION	non-polymer		39.1	6	Chemie (K)
6	D, F, B	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
7	D, F, B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	3	Chemie (GDP)
8	D, F, B	ALUMINUM FLUORIDE	non-polymer		84.0	3	Chemie (AF3)
9		water	water		18.0	34	Chemie (HOH)

Sequence modifications

A, C, E: 326 - 623 (UniProt: Q5ZSM7)

PDB	External Database	Details
Ser 325	-	EXPRESSION TAG

B, D, F: 4 - 177 (UniProt: P62820)

PDB	External Database	Details
Mse -6	-	EXPRESSION TAG
Gly -5	-	EXPRESSION TAG
His -4	-	EXPRESSION TAG
His -3	-	EXPRESSION TAG
His -2	-	EXPRESSION TAG
His -1	-	EXPRESSION TAG
His 0	-	EXPRESSION TAG
His 1	-	EXPRESSION TAG
Gly 2	-	EXPRESSION TAG
Ser 3	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	165274.4
Non-Polymers*	Number of molecules	35
	Total formula weight	3334.3
All*	Total formula weight	168608.8

*Water molecules are not included.