4IRU
Crystal Structure of lepB GAP core in a transition state mimetic complex with Rab1A and ALF3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791, 0.9639 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 139.404, 139.404, 384.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.010 - 3.200 |
| R-factor | 0.23247 |
| Rwork | 0.230 |
| R-free | 0.28812 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.445 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol (in Phenix) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.283 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 30171 | |
| <I/σ(I)> | 3.8 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 12% PEG4000, 0.1M Hepes, 0.2M potassium acetate, 0.002M aluminium chloride, 0.02M sodium fluoride, 0.01M 2-mercaptoethanol, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
