4HN1
Crystal Structure of H60N/Y130F double mutant of ChmJ, a 3'-monoepimerase from Streptomyces bikiniensis in complex with dTDP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Putative 3-epimerase in D-allose pathway | polymer | 201 | 22323.8 | 4 | UniProt (Q5SFD1) Pfam (PF00908) In PDB | Streptomyces bikiniensis | |
2 | A, B, C, D | 1,2-ETHANEDIOL | non-polymer | 62.1 | 11 | Chemie (EDO) | |||
3 | A, B, C | THYMIDINE-5'-DIPHOSPHATE | non-polymer | 402.2 | 4 | Chemie (TYD) | |||
4 | A, B | THYMIDINE | non-polymer | 242.2 | 2 | Chemie (THM) | |||
5 | C, D | THYMINE | non-polymer | 126.1 | 2 | Chemie (TDR) | |||
6 | water | water | 18.0 | 419 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 196 (UniProt: Q5SFD1)
PDB | External Database | Details |
---|---|---|
Asn 60 | His 60 | engineered mutation |
Phe 130 | Tyr 130 | engineered mutation |
Leu 197 | - | expression tag |
Glu 198 | - | expression tag |
His 199 | - | expression tag |
His 200 | - | expression tag |
His 201 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 89295.4 | |
Non-Polymers* | Number of molecules | 19 |
Total formula weight | 3028.2 | |
All* | Total formula weight | 92323.5 |