4HN1
Crystal Structure of H60N/Y130F double mutant of ChmJ, a 3'-monoepimerase from Streptomyces bikiniensis in complex with dTDP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Putative 3-epimerase in D-allose pathway | polymer | 201 | 22323.8 | 4 | UniProt (Q5SFD1) | Streptomyces bikiniensis | |
| 2 | E, F, G, H, K... (A, B, C, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 11 | Chemie (EDO) | |||
| 3 | I, N, R, S (A, B, C) | THYMIDINE-5'-DIPHOSPHATE | non-polymer | 402.2 | 4 | Chemie (TYD) | |||
| 4 | J, O (A, B) | THYMIDINE | non-polymer | 242.2 | 2 | Chemie (THM) PubChem (1134) PubChem (5789) PubChem (72247) PubChem (159269) PubChem (448456) PubChem (451756) PubChem (453555) PubChem (489518) PubChem (638041) PubChem (7048808) PubChem (44381956) PubChem (46782983) PubChem (3000346) PubChem (5270603) PubChem (57543820) PubChem (57548467) PubChem (58445182) PubChem (59119194) PubChem (68231510) PubChem (68441874) PubChem (68642271) PubChem (68642272) PubChem (121354259) PubChem (142978968) PubChem (144640942) PubChem (163655970) | |||
| 5 | T, W (C, D) | THYMINE | non-polymer | 126.1 | 2 | Chemie (TDR) PubChem (1135) | |||
| 6 | AA, X, Y, Z (D, A, B, C) | water | water | 18.0 | 419 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 196 (UniProt: Q5SFD1)
| PDB | External Database | Details |
|---|---|---|
| Asn 60 | His 60 | engineered mutation |
| Phe 130 | Tyr 130 | engineered mutation |
| Leu 197 | - | expression tag |
| Glu 198 | - | expression tag |
| His 199 | - | expression tag |
| His 200 | - | expression tag |
| His 201 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 89295.4 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 3028.2 | |
| All* | Total formula weight | 92323.5 |
