4HN1

Crystal Structure of H60N/Y130F double mutant of ChmJ, a 3'-monoepimerase from Streptomyces bikiniensis in complex with dTDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Putative 3-epimerase in D-allose pathway	polymer	201	22323.8	4	UniProt (Q5SFD1) Pfam (PF00908)	Streptomyces bikiniensis
2	E, F, G, H, K... (A, B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
3	I, N, R, S (A, B, C)	THYMIDINE-5'-DIPHOSPHATE	non-polymer		402.2	4	Chemie (TYD)
4	J, O (A, B)	THYMIDINE	non-polymer		242.2	2	Chemie (THM)
5	T, W (C, D)	THYMINE	non-polymer		126.1	2	Chemie (TDR)
6	AA, X, Y, Z (D, A, B, C)	water	water		18.0	419	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 196 (UniProt: Q5SFD1)

PDB	External Database	Details
Asn 60	His 60	engineered mutation
Phe 130	Tyr 130	engineered mutation
Leu 197	-	expression tag
Glu 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag
His 201	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	89295.4
Non-Polymers*	Number of molecules	19
	Total formula weight	3028.2
All*	Total formula weight	92323.5

*Water molecules are not included.