4GZM

Crystal structure of RAC1 F28L mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ras-related C3 botulinum toxin substrate 1	polymer	204	22700.0	2	UniProt (P63000) Pfam (PF00071)	Homo sapiens (human)	RAC1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1
2	C, E (A, B)	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE	non-polymer		539.2	2	Chemie (GSP)
3	D, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 2 - 177 (UniProt: P63000)

PDB	External Database	Details
Met -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Ser -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Leu -13	-	expression tag
Val -12	-	expression tag
Pro -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
Met -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag
Leu 28	Phe 28	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45400.1
Non-Polymers*	Number of molecules	4
	Total formula weight	1127.1
All*	Total formula weight	46527.2

*Water molecules are not included.