4GZM
Crystal structure of RAC1 F28L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.094, 98.807, 51.994 |
| Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
| Resolution | 27.823 - 2.800 |
| R-factor | 0.25533 |
| Rwork | 0.254 |
| R-free | 0.28805 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3th5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.983 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.823 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.119 | 0.578 |
| Number of reflections | 10037 | |
| <I/σ(I)> | 7.591 | 2.4 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 16-18% PEG4000, 8% isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
