4GZL

Crystal structure of RAC1 Q61L mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ras-related C3 botulinum toxin substrate 1	polymer	204	22719.1	2	UniProt (P63000) Pfam (PF00071)	Homo sapiens (human)	RAC1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1
2	C, F (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	2	Chemie (GNP)
3	D, G (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	E (A)	ISOPROPYL ALCOHOL	non-polymer		60.1	1	Chemie (IPA)
5	H, I (A, B)	water	water		18.0	156	Chemie (HOH)

Sequence modifications

A, B: 2 - 177 (UniProt: P63000)

PDB	External Database	Details
Met -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Ser -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Leu -13	-	expression tag
Val -12	-	expression tag
Pro -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
Met -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag
Leu 61	Gln 61	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45438.1
Non-Polymers*	Number of molecules	5
	Total formula weight	1153.1
All*	Total formula weight	46591.2

*Water molecules are not included.