4GZL
Crystal structure of RAC1 Q61L mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.07500 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.830, 72.940, 91.597 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.18937 |
Rwork | 0.186 |
R-free | 0.25021 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3th5 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.024 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.110 | 0.625 |
Number of reflections | 23219 | |
<I/σ(I)> | 14.244 | 3.541 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 6.6 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 16-18% PEG4000, 6% isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |