4GKJ

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a human mitochondrial anticodon stem loop (ASL) of transfer RNA Methionine (TRNAMET) bound to an mRNA with an AUG-codon in the A-site and paromomycin.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	16S rRNA	polymer	1513	491166.8	1	GenBank (48256)	Thermus thermophilus
2	B (B)	30S ribosomal protein S2	polymer	234	26987.3	1	UniProt (P80371) Pfam (PF00318)	Thermus thermophilus
3	C (C)	30S ribosomal protein S3	polymer	206	22862.4	1	UniProt (P80372) Pfam (PF07650) Pfam (PF00189)	Thermus thermophilus
4	D (D)	30S ribosomal protein S4	polymer	208	24242.3	1	UniProt (P80373) Pfam (PF00163) Pfam (PF01479)	Thermus thermophilus
5	E (E)	30S ribosomal protein S5	polymer	150	16331.1	1	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719)	Thermus thermophilus
6	F (F)	30S ribosomal protein S6	polymer	101	11988.8	1	UniProt (Q5SLP8) Pfam (PF01250)	Thermus thermophilus	TS9
7	G (G)	30S ribosomal protein S7	polymer	155	17919.8	1	UniProt (P17291) Pfam (PF00177)	Thermus thermophilus
8	H (H)	30S ribosomal protein S8	polymer	138	15868.6	1	UniProt (Q5SHQ2)	Thermus thermophilus
9	I (I)	30S ribosomal protein S9	polymer	127	14298.5	1	UniProt (P80374) Pfam (PF00380)	Thermus thermophilus
10	J (J)	30S ribosomal protein S10	polymer	98	11299.2	1	UniProt (Q5SHN7) Pfam (PF00338)	Thermus thermophilus
11	K (K)	30S ribosomal protein S11	polymer	119	12606.4	1	UniProt (P80376) Pfam (PF00411)	Thermus thermophilus
12	L (L)	30S ribosomal protein S12	polymer	124	13804.3	1	UniProt (Q5SHN3) Pfam (PF00164)	Thermus thermophilus
13	M (M)	30S ribosomal protein S13	polymer	125	14207.7	1	UniProt (P80377) Pfam (PF00416)	Thermus thermophilus
14	N (N)	30S ribosomal protein S14 type Z	polymer	60	7027.5	1	UniProt (Q5SHQ1)	Thermus thermophilus
15	O (O)	30S ribosomal protein S15	polymer	88	10447.2	1	UniProt (Q5SJ76) Pfam (PF00312)	Thermus thermophilus
16	P (P)	30S ribosomal protein S16	polymer	83	9924.5	1	UniProt (Q5SJH3) Pfam (PF00886)	Thermus thermophilus
17	Q (Q)	30S ribosomal protein S17	polymer	104	12193.5	1	UniProt (Q5SHP7)	Thermus thermophilus
18	R (R)	30S ribosomal protein S18	polymer	73	8483.2	1	UniProt (Q5SLQ0) Pfam (PF01084)	Thermus thermophilus
19	S (S)	30S ribosomal protein S19	polymer	80	9203.7	1	UniProt (Q5SHP2) Pfam (PF00203)	Thermus thermophilus
20	T (T)	30S ribosomal protein S20	polymer	99	10907.1	1	UniProt (P80380) Pfam (PF01649)	Thermus thermophilus
21	U (V)	30S ribosomal protein Thx	polymer	24	2960.5	1	UniProt (Q5SIH3) Pfam (PF17070)	Thermus thermophilus	S31
22	V (W)	mRNA A-site fragment	polymer	6	1923.2	1		Synthetic
23	W (X)	tRNA ASL human mitochondrial Met	polymer	17	5405.3	1		Synthetic
24	AA, AB, AC, AD, AE... (A, B, N)	MAGNESIUM ION	non-polymer		24.3	186	Chemie (MG)
25	ZG (A)	PAROMOMYCIN	non-polymer		615.6	1	Chemie (PAR)
26	BH, DH (D, N)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 5 - 1521 (GenBank: 48256)

PDB	External Database	Details
C 1005	-	insertion
G 1013	-	insertion
C 1225	-	insertion
A 1226	-	insertion
U 1517	C 1508	conflict
U 1519	C 1510	conflict

I: 2 - 128 (UniProt: P80374)

PDB	External Database	Details
Arg 58	His 58	conflict

Q: 2 - 105 (UniProt: Q5SHP7)

PDB	External Database	Details
Gln 96	Glu 96	conflict

R: 16 - 88 (UniProt: Q5SLQ0)

PDB	External Database	Details
Gly 60	Ala 60	conflict

T: 8 - 106 (UniProt: P80380)

PDB	External Database	Details
Val 41	Ile 41	conflict

Contents of the asymmetric unit

Polymers	Number of chains	23
	Total formula weight	772058.6
Non-Polymers*	Number of molecules	189
	Total formula weight	5267.2
All*	Total formula weight	777325.7

*Water molecules are not included.