4GKJ

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a human mitochondrial anticodon stem loop (ASL) of transfer RNA Methionine (TRNAMET) bound to an mRNA with an AUG-codon in the A-site and paromomycin.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	400.550, 400.550, 176.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.148 - 3.298
R-factor	0.19
Rwork	0.188
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.354
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	283.000		3.040
High resolution limit [Å]	3.000	9.620	2.900
Rmerge		0.057	9.636
Number of reflections	276947
<I/σ(I)>		13.26	0.21
Completeness [%]	62.3	77.9	98.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate, pH 6.5 VAPOR DIFFUSION, HANGING DROP, temperature 277K, temperature 277.0K