4FLO
Crystal structure of Amylosucrase double mutant A289P-F290C from Neisseria polysaccharea
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Amylosucrase | polymer | 628 | 71546.1 | 1 | UniProt (Q9ZEU2) Pfam (PF00128) Pfam (PF22582) | Neisseria polysaccharea | |
| 2 | B (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 3 | C, D, E (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 4 | F (A) | HEXAETHYLENE GLYCOL | non-polymer | 282.3 | 1 | Chemie (P6G) | |||
| 5 | G (A) | water | water | 18.0 | 578 | Chemie (HOH) |
Sequence modifications
A: 5 - 628 (UniProt: Q9ZEU2)
| PDB | External Database | Details |
|---|---|---|
| Ser 1 | - | expression tag |
| Pro 2 | - | expression tag |
| Asn 3 | - | expression tag |
| Ser 4 | - | expression tag |
| Pro 289 | Ala 297 | engineered mutation |
| Cys 290 | Phe 298 | engineered mutation |
| Asp 537 | Gly 545 | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 71546.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 680.8 | |
| All* | Total formula weight | 72226.8 |
