4FLO

Crystal structure of Amylosucrase double mutant A289P-F290C from Neisseria polysaccharea

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0005975	biological_process	carbohydrate metabolic process
A	0016757	molecular_function	glycosyltransferase activity
A	0047669	molecular_function	amylosucrase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE TRS A 701

Chain	Residue
A	ASP144
A	HOH814
A	TYR147
A	HIS187
A	PHE229
A	PHE250
A	ASP286
A	GLU328
A	ARG509
A	HOH800

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 702

Chain	Residue
A	TYR375
A	ARG376
A	ASP403
A	HOH1273

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 703

Chain	Residue
A	PRO203
A	ASP526
A	HOH1254
A	HOH1334

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 704

Chain	Residue
A	ARG226
A	PHE250
A	CYS290
A	GLU300
A	GLU328
A	ILE330

---

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE P6G A 705

Chain	Residue
A	LEU204
A	PHE205
A	TRP263
A	ARG266
A	ALA267
A	HOH916
A	HOH1262

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:15023061, ECO:0000305|PubMed:10828446

Chain	Residue	Details
A	ASP286

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000305|PubMed:10828446

Chain	Residue	Details
A	GLU328

---

site_id	SWS_FT_FI3
Number of Residues	7
Details	BINDING: BINDING => ECO:0000305|PubMed:11467966, ECO:0000305|PubMed:12364331, ECO:0000305|PubMed:15023061

Chain	Residue	Details
A	ASP144
A	HIS187
A	GLN254
A	ARG284
A	HIS392
A	ASP393
A	ARG509

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	SITE: Transition state stabilizer => ECO:0000250

Chain	Residue	Details
A	ASP444

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