4FLO
Crystal structure of Amylosucrase double mutant A289P-F290C from Neisseria polysaccharea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.93340 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.030, 115.980, 60.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.990 - 2.200 |
R-factor | 0.1691 |
Rwork | 0.164 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g5a |
RMSD bond length | 0.007 |
RMSD bond angle | 1.067 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.660 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.101 | 0.306 |
Number of reflections | 35028 | |
<I/σ(I)> | 7.2 | 2.5 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 4.7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | 30% PEG 6000, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K |