4FJV

Crystal Structure of Human Otubain2 and Ubiquitin Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ubiquitin thioesterase OTUB2	polymer	237	27533.2	2	UniProt (Q96DC9) Pfam (PF10275)	Homo sapiens (human)	Deubiquitinating enzyme OTUB2, OTU domain-containing ubiquitin aldehyde-binding protein 2, Otubain-2, Ubiquitin-specific-processing protease OTUB2
2	B, D (B, D)	Polyubiquitin-C	polymer	86	9799.1	2	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (human)	Ubiquitin
3	E, F, G, I, J... (A, C)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
4	H, L (B, D)	ETHANAMINE	non-polymer		45.1	2	Chemie (NEH)
5	M, N, O, P (A, B, C, D)	water	water		18.0	560	Chemie (HOH)

Sequence modifications

A, C: 1 - 234 (UniProt: Q96DC9)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

B, D: 1 - 76 (UniProt: P0CG48)

PDB	External Database	Details
Tyr -9	-	expression tag
Pro -8	-	expression tag
Tyr -7	-	expression tag
Asp -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Asp -3	-	expression tag
Tyr -2	-	expression tag
Ala -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	74664.6
Non-Polymers*	Number of molecules	8
	Total formula weight	642.7
All*	Total formula weight	75307.4

*Water molecules are not included.