4FFB

A TOG:alpha/beta-tubulin Complex Structure Reveals Conformation-Based Mechanisms For a Microtubule Polymerase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Tubulin alpha-1 chain	polymer	447	49853.9	1	UniProt (P09733) Pfam (PF00091) Pfam (PF03953)	Saccharomyces cerevisiae (Baker's yeast)
2	B (B)	Tubulin beta chain	polymer	463	51910.5	1	UniProt (P02557) Pfam (PF00091) Pfam (PF03953)	Saccharomyces cerevisiae (Baker's yeast)	Beta-tubulin
3	C (C)	Protein STU2	polymer	278	31666.2	1	UniProt (P46675) Pfam (PF21041)	Saccharomyces cerevisiae (Baker's yeast)	Suppressor of tubulin 2
4	D, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	E, G (A, B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)

Sequence modifications

B: 1 - 457 (UniProt: P02557)

PDB	External Database	Details
Arg 175	Thr 175	engineered mutation
Arg 179	Val 179	engineered mutation
His 458	-	expression tag
His 459	-	expression tag
His 460	-	expression tag
His 461	-	expression tag
His 462	-	expression tag
His 463	-	expression tag

C: 1 - 272 (UniProt: P46675)

PDB	External Database	Details
His 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag
His 276	-	expression tag
His 277	-	expression tag
His 278	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	133430.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1095.0
All*	Total formula weight	134525.5

*Water molecules are not included.