4FFB

A TOG:alpha/beta-tubulin Complex Structure Reveals Conformation-Based Mechanisms For a Microtubule Polymerase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Tubulin alpha-1 chain	polymer	447	49853.9	1	UniProt (P09733) Pfam (PF00091) Pfam (PF03953)	Saccharomyces cerevisiae (Baker's yeast)
2	B (B)	Tubulin beta chain	polymer	463	51910.5	1	UniProt (P02557) Pfam (PF00091) Pfam (PF03953)	Saccharomyces cerevisiae (Baker's yeast)	Beta-tubulin
3	C (C)	Protein STU2	polymer	278	31666.2	1	UniProt (P46675) Pfam (PF21041)	Saccharomyces cerevisiae (Baker's yeast)	Suppressor of tubulin 2
4	D, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
5	E, G (A, B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)

Sequence modifications

B: 1 - 457 (UniProt: P02557)

PDB	External Database	Details
Arg 175	Thr 175	engineered mutation
Arg 179	Val 179	engineered mutation
His 458	-	expression tag
His 459	-	expression tag
His 460	-	expression tag
His 461	-	expression tag
His 462	-	expression tag
His 463	-	expression tag

C: 1 - 272 (UniProt: P46675)

PDB	External Database	Details
His 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag
His 276	-	expression tag
His 277	-	expression tag
His 278	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	133430.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1095.0
All*	Total formula weight	134525.5

*Water molecules are not included.