4FFB
A TOG:alpha/beta-tubulin Complex Structure Reveals Conformation-Based Mechanisms For a Microtubule Polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.120, 98.040, 91.366 |
| Unit cell angles | 90.00, 100.31, 90.00 |
Refinement procedure
| Resolution | 44.945 - 2.882 |
| R-factor | 0.2099 |
| Rwork | 0.207 |
| R-free | 0.26730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SA0 CHAIN A 1SA0 CHAIN B 2qk1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.697 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1063)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.967 | |
| Number of reflections | 39347 | |
| <I/σ(I)> | 0.9 | |
| Completeness [%] | 86.0 | 22.6 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 298 | 20% (v/v) PEG 400, 0.1 M MES pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
