4F5C
Crystal structure of the spike receptor binding domain of a porcine respiratory coronavirus in complex with the pig aminopeptidase N ectodomain
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Aminopeptidase N | polymer | 959 | 108897.0 | 2 | UniProt (P15145) Pfam (PF17900) Pfam (PF01433) Pfam (PF11838) | Sus scrofa (pig) | AP-N, pAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, gp130 |
| 2 | C, D (E, F) | PRCV spike protein | polymer | 440 | 48365.9 | 2 | UniProt (Q84852) Pfam (PF01600) | Porcine respiratory coronavirus (PRCV) | S protein |
| 3 | E, F, G (C, D, G) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 4 | H, R (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 5 | AA, BA, CA, I, J... (B, A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 20 | Chemie (NAG) | |||
| 6 | DA, EA (A, B) | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A, B: 36 - 963 (UniProt: P15145)
E, F: 1 - 426 (UniProt: Q84852)
| PDB | External Database | Details |
|---|---|---|
| Tyr 19 | - | expression tag |
| Pro 20 | - | expression tag |
| Tyr 21 | - | expression tag |
| Asp 22 | - | expression tag |
| Val 23 | - | expression tag |
| Pro 24 | - | expression tag |
| Asp 25 | - | expression tag |
| Tyr 26 | - | expression tag |
| Ala 27 | - | expression tag |
| Gly 28 | - | expression tag |
| Ala 29 | - | expression tag |
| Gln 30 | - | expression tag |
| Pro 31 | - | expression tag |
| Ala 32 | - | expression tag |
| Arg 33 | - | expression tag |
| Ser 34 | - | expression tag |
| Pro 35 | - | expression tag |
| Asn 82 | Phe 82 | conflict |
| Phe 107 | Leu 107 | conflict |
| Ile 108 | Leu 108 | conflict |
| Leu 964 | - | expression tag |
| Val 965 | - | expression tag |
| Pro 966 | - | expression tag |
| Arg 967 | - | expression tag |
| Gly 968 | - | expression tag |
| Ser 969 | - | expression tag |
| Asp 970 | - | expression tag |
| Tyr 971 | - | expression tag |
| Lys 972 | - | expression tag |
| Asp 973 | - | expression tag |
| Asp 974 | - | expression tag |
| Asp 975 | - | expression tag |
| Asp 976 | - | expression tag |
| Lys 977 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp 409 | Glu 425 | conflict |
| Leu 427 | - | expression tag |
| Val 428 | - | expression tag |
| Pro 429 | - | expression tag |
| Arg 430 | - | expression tag |
| Gly 431 | - | expression tag |
| Ser 432 | - | expression tag |
| Asp 433 | - | expression tag |
| Tyr 434 | - | expression tag |
| Lys 435 | - | expression tag |
| Asp 436 | - | expression tag |
| Asp 437 | - | expression tag |
| Asp 438 | - | expression tag |
| Asp 439 | - | expression tag |
| Lys 440 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 314525.7 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1273.2 | |
| Non-Polymers* | Number of molecules | 22 |
| Total formula weight | 4555.0 | |
| All* | Total formula weight | 320353.9 |
