4F5C

Crystal structure of the spike receptor binding domain of a porcine respiratory coronavirus in complex with the pig aminopeptidase N ectodomain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Aminopeptidase N	polymer	959	108897.0	2	UniProt (P15145) Pfam (PF17900) Pfam (PF01433) Pfam (PF11838) In PDB	Sus scrofa (pig)	AP-N, pAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, gp130
2	E, F	PRCV spike protein	polymer	440	48365.9	2	UniProt (Q84852) Pfam (PF01600) In PDB	Porcine respiratory coronavirus (PRCV)	S protein
3	C, D, G	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
4	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	B, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	20	Chemie (NAG)
6		water	water		18.0	4	Chemie (HOH)

Sequence modifications

A, B: 36 - 963 (UniProt: P15145)

PDB	External Database	Details
Tyr 19	-	expression tag
Pro 20	-	expression tag
Tyr 21	-	expression tag
Asp 22	-	expression tag
Val 23	-	expression tag
Pro 24	-	expression tag
Asp 25	-	expression tag
Tyr 26	-	expression tag
Ala 27	-	expression tag
Gly 28	-	expression tag
Ala 29	-	expression tag
Gln 30	-	expression tag
Pro 31	-	expression tag
Ala 32	-	expression tag
Arg 33	-	expression tag
Ser 34	-	expression tag
Pro 35	-	expression tag
Asn 82	Phe 82	conflict
Phe 107	Leu 107	conflict
Ile 108	Leu 108	conflict
Leu 964	-	expression tag
Val 965	-	expression tag
Pro 966	-	expression tag
Arg 967	-	expression tag
Gly 968	-	expression tag
Ser 969	-	expression tag
Asp 970	-	expression tag
Tyr 971	-	expression tag
Lys 972	-	expression tag
Asp 973	-	expression tag
Asp 974	-	expression tag
Asp 975	-	expression tag
Asp 976	-	expression tag
Lys 977	-	expression tag

E, F: 1 - 426 (UniProt: Q84852)

PDB	External Database	Details
Asp 409	Glu 425	conflict
Leu 427	-	expression tag
Val 428	-	expression tag
Pro 429	-	expression tag
Arg 430	-	expression tag
Gly 431	-	expression tag
Ser 432	-	expression tag
Asp 433	-	expression tag
Tyr 434	-	expression tag
Lys 435	-	expression tag
Asp 436	-	expression tag
Asp 437	-	expression tag
Asp 438	-	expression tag
Asp 439	-	expression tag
Lys 440	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	314525.7
Branched	Number of molecules	3
	Total formula weight	1273.2
Non-Polymers*	Number of molecules	22
	Total formula weight	4555.0
All*	Total formula weight	320353.9

*Water molecules are not included.