4F5C
Crystal structure of the spike receptor binding domain of a porcine respiratory coronavirus in complex with the pig aminopeptidase N ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 220.859, 87.938, 176.912 |
Unit cell angles | 90.00, 90.54, 90.00 |
Refinement procedure
Resolution | 24.912 - 3.200 |
R-factor | 0.2034 |
Rwork | 0.201 |
R-free | 0.24490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.900 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.057 | 0.377 |
Number of reflections | 54019 | |
<I/σ(I)> | 13.2 | 2.1 |
Completeness [%] | 96.0 | 97.2 |
Redundancy | 3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 20% PEG4000, 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |