4F02

Crystal structure of the PABP-binding site of eIF4G in complex with RRM1-2 of PABP and poly(A)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Polyadenylate-binding protein 1	polymer	213	23833.1	2	UniProt (P11940) Pfam (PF00076)	Homo sapiens (human)	PABP-1, Poly(A)-binding protein 1
2	B, E (B, E)	RNA (5'-R(*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3')	polymer	11	3576.3	2
3	C, F (C, F)	Eukaryotic translation initiation factor 4 gamma 1	polymer	27	3026.4	2	UniProt (Q04637)	Homo sapiens	eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220
4	G, H, I, J, K (A, B, C, D, E)	water	water		18.0	196	Chemie (HOH)

Sequence modifications

A, D: 1 - 190 (UniProt: P11940)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Tyr 191	-	expression tag
Pro 192	-	expression tag
Tyr 193	-	expression tag
Asp 194	-	expression tag
Val 195	-	expression tag
Pro 196	-	expression tag
Asp 197	-	expression tag
Tyr 198	-	expression tag
Ala 199	-	expression tag
Gly 200	-	expression tag
Ser 201	-	expression tag
Ser 202	-	expression tag
Gly 203	-	expression tag
Arg 204	-	expression tag
Ile 205	-	expression tag
Val 206	-	expression tag
Thr 207	-	expression tag
Asp 208	-	expression tag

C, F: 178 - 203 (UniProt: Q04637)

PDB	External Database	Details
Tyr 204	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	60871.7
All*	Total formula weight	60871.7

*Water molecules are not included.