4F02
Crystal structure of the PABP-binding site of eIF4G in complex with RRM1-2 of PABP and poly(A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2010-05-29 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 130.191, 130.191, 86.872 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.110 - 2.000 |
R-factor | 0.20541 |
Rwork | 0.204 |
R-free | 0.23016 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 2.123 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.110 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 47356 | |
Completeness [%] | 97.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.03 M Tris, 1.3 M (NH4)2SO4, 5% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |