4EZM

Crystal structure of the human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Ig epsilon chain C region	polymer	223	24920.1	6	UniProt (P01854) Pfam (PF07654) In PDB	Homo sapiens (human)
2	G, H, I, J, K...	Low affinity immunoglobulin epsilon Fc receptor	polymer	143	16165.0	6	UniProt (P06734) Pfam (PF00059) In PDB	Homo sapiens (human)	BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, Low affinity immunoglobulin epsilon Fc receptor membrane-bound form, Low affinity immunoglobulin epsilon Fc receptor soluble form
3	M, N, O, P, Q...	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	6	In PDB GlyTouCan (G22768VO)
4	C	alpha-D-mannopyranose	non-polymer		180.2	2	Chemie (MAN)
5		water	water		18.0	1	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 328 - 547 (UniProt: P01854)

PDB	External Database	Details
Ala 325	-	expression tag
Asp 326	-	expression tag
Pro 327	-	expression tag
Gln 371	Asn 252	engineered mutation
Gln 383	Asn 264	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	246510.8
Branched	Number of molecules	6
	Total formula weight	5464.9
Non-Polymers*	Number of molecules	2
	Total formula weight	360.3
All*	Total formula weight	252336.0

*Water molecules are not included.