4EZM

Crystal structure of the human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	62.890, 110.750, 376.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.870 - 3.100
R-factor	0.2649
Rwork	0.264
R-free	0.28310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h9z 2h2r
RMSD bond length	0.041
RMSD bond angle	1.290
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.870
High resolution limit [Å]	3.100
Number of reflections	48290
Completeness [%]	92.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	3% PEG 8,000, 0.1 M Tris-HCl pH 7.5, VAPOR DIFFUSION, temperature 298K