4EKC
Structure of human regulator of G protein signaling 2 (RGS2) in complex with murine Galpha-q(R183C)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Guanine nucleotide-binding protein G(q) subunit alpha | polymer | 347 | 40634.2 | 2 | UniProt (P21279) Pfam (PF00503) | Mus musculus (mouse) | Guanine nucleotide-binding protein alpha-q |
| 2 | B, D (B, D) | Regulator of G-protein signaling 2 | polymer | 137 | 15871.8 | 2 | UniProt (P41220) Pfam (PF00615) | Homo sapiens (human) | RGS2, Cell growth-inhibiting gene 31 protein, G0/G1 switch regulatory protein 8 |
| 3 | E, H (A, C) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 4 | F, I (A, C) | TETRAFLUOROALUMINATE ION | non-polymer | 103.0 | 2 | Chemie (ALF) PubChem (5311325) | |||
| 5 | G, J (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 6 | K, L (A, C) | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A, C: 18 - 359 (UniProt: P21279)
B, D: 72 - 203 (UniProt: P41220)
| PDB | External Database | Details |
|---|---|---|
| Gly 13 | - | expression tag |
| Ala 14 | - | expression tag |
| Met 15 | - | expression tag |
| Gly 16 | - | expression tag |
| Ser 17 | - | expression tag |
| Asp 125 | Glu 125 | engineered mutation |
| Val 126 | Asn 126 | engineered mutation |
| Asp 128 | Tyr 128 | engineered mutation |
| Tyr 129 | Val 129 | engineered mutation |
| Ala 130 | Asp 130 | engineered mutation |
| Cys 183 | Arg 183 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 67 | - | expression tag |
| Glu 68 | - | expression tag |
| Phe 69 | - | expression tag |
| Gly 70 | - | expression tag |
| Ser 71 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 113012.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1141.0 | |
| All* | Total formula weight | 114153.0 |
