4EKC
Structure of human regulator of G protein signaling 2 (RGS2) in complex with murine Galpha-q(R183C)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-03-18 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.603, 124.983, 97.196 |
| Unit cell angles | 90.00, 124.13, 90.00 |
Refinement procedure
| Resolution | 19.980 - 7.400 |
| R-factor | 0.16084 |
| Rwork | 0.158 |
| R-free | 0.22037 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2AF0 and 2BCJ |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 7.530 |
| High resolution limit [Å] | 7.400 | 7.400 |
| Number of reflections | 1820 | |
| <I/σ(I)> | 9.1 | 2.5 |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 17% PEG 3350, 200 mM NaCl, and 100 mM MES pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
