4EB6

Tubulin-Vinblastine: Stathmin-like complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Tubulin alpha chain	polymer	451	50204.4	2	UniProt (D0VWZ0) Pfam (PF00091) Pfam (PF03953) In PDB	Ovis aries (sheep)
2	B, D	Tubulin beta chain	polymer	445	50043.9	2	UniProt (D0VWY9) Pfam (PF00091) Pfam (PF03953) In PDB	Ovis aries (domestic sheep lambs wild sheep)
3	E	Stathmin-4	polymer	142	16719.9	1	UniProt (P63043) Pfam (PF00836) In PDB	Rattus norvegicus (rat)	Stathmin-like protein B3, RB3
4	A, C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
5	A, C	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	A, B, D	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
7	B, D	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
8	C	(2ALPHA,2'BETA,3BETA,4ALPHA,5BETA)-VINCALEUKOBLASTINE	non-polymer		811.0	1	Chemie (VLB)
9		water	water		18.0	31	Chemie (HOH)

Sequence modifications

A, C: 1 - 451 (UniProt: D0VWZ0)

PDB	External Database	Details
Ser 232	Gly 232	SEE REMARK 999
Ser 340	Thr 340	SEE REMARK 999

B, D: 1 - 455 (UniProt: D0VWY9)

PDB	External Database	Details
Thr 317	Ala 315	SEE REMARK 999
Ile 318	Val 316	SEE REMARK 999
Ile 335	Val 333	SEE REMARK 999
Ser 375	Ala 365	SEE REMARK 999

E: 5 - 145 (UniProt: P63043)

PDB	External Database	Details
Ala 4	-	expression tag
Ala 14	Cys 58	engineered mutation
Trp 20	Phe 64	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	217216.6
Non-Polymers*	Number of molecules	12
	Total formula weight	3272.7
All*	Total formula weight	220489.2

*Water molecules are not included.