4EB6
Tubulin-Vinblastine: Stathmin-like complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-10 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.690, 129.680, 252.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.060 - 3.470 |
R-factor | 0.2152 |
Rwork | 0.213 |
R-free | 0.25330 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.230 | 3.650 |
High resolution limit [Å] | 3.470 | 3.470 |
Rmerge | 0.075 | 0.685 |
Number of reflections | 28253 | |
<I/σ(I)> | 12.2 | 1.8 |
Completeness [%] | 99.2 | 98.7 |
Redundancy | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 293 | PEG, Li2SO4, PIPES, pH 6.80, VAPOR DIFFUSION, HANGING DROP, temperature 293K |