4EAJ

Co-crystal of AMPK core with AMP soaked with ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	5'-AMP-activated protein kinase catalytic subunit alpha-1	polymer	106	12097.9	1	UniProt (P54645) Pfam (PF16579)	Rattus norvegicus (Rat)	AMPK subunit alpha-1,Acetyl-CoA carboxylase kinase,ACACA kinase,Hydroxymethylglutaryl-CoA reductase kinase,HMGCR kinase,Tau-protein kinase PRKAA1
2	B (B)	5'-AMP-activated protein kinase subunit beta-2	polymer	85	9886.6	1	UniProt (O43741) Pfam (PF04739)	Homo sapiens (Human)	AMPK subunit beta-2
3	C (C)	5'-AMP-activated protein kinase subunit gamma-1	polymer	330	37434.1	1	UniProt (P80385) Pfam (PF00571)	Rattus norvegicus (Rat)	AMPKg
4	D, E (C)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
5	F (C)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)
6	G, H, I (A, B, C)	water	water		18.0	54	Chemie (HOH)

Sequence modifications

A: 394 - 468 (UniProt: P54645)

PDB	External Database	Details
Gly 389	-	expression tag
Pro 390	-	expression tag
His 391	-	expression tag
Met 392	-	expression tag
Gly 393	-	expression tag

A: 475 - 494 (UniProt: P54645)

PDB	External Database	Details
Gly 469	-	linker
Gly 470	-	linker
Gly 471	-	linker
Gly 472	-	linker
Gly 473	-	linker
Gly 474	-	linker

B: 189 - 272 (UniProt: O43741)

PDB	External Database	Details
Met 188	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	59418.6
Non-Polymers*	Number of molecules	3
	Total formula weight	1361.6
All*	Total formula weight	60780.2

*Water molecules are not included.