4EA4

Structure of the glycosylase domain of MBD4 bound to 5hmU-containing DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Methyl-CpG-binding domain protein 4	polymer	155	18713.6	1	UniProt (O95243)	Homo sapiens (human)	Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase
2	B (C)	DNA (5'-D(*CP*CP*AP*GP*CP*GP*(5HU)P*GP*CP*AP*GP*C)-3')	polymer	12	3664.4	1
3	C (D)	DNA (5'-D(*GP*CP*TP*GP*CP*GP*CP*GP*CP*TP*GP*G)-3')	polymer	12	3695.4	1
4	D, E, F (A, C, D)	water	water		18.0	82	Chemie (HOH)

Sequence modifications

A: 427 - 574 (UniProt: O95243)

PDB	External Database	Details
Met 426	-	expression tag
Ala 560	Asp 560	engineered mutation
His 575	-	expression tag
His 576	-	expression tag
His 577	-	expression tag
His 578	-	expression tag
His 579	-	expression tag
His 580	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	26073.4
All*	Total formula weight	26073.4

*Water molecules are not included.