4EA4
Structure of the glycosylase domain of MBD4 bound to 5hmU-containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | .9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.270, 63.180, 94.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.910 - 2.000 |
| R-factor | 0.2263 |
| Rwork | 0.224 |
| R-free | 0.26470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e9e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 16435 | |
| Completeness [%] | 96.3 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 20% PEG 4000, 0.1 M Nacitrate, 20% isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
