4EA4
Structure of the glycosylase domain of MBD4 bound to 5hmU-containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | .9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.270, 63.180, 94.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.910 - 2.000 |
R-factor | 0.2263 |
Rwork | 0.224 |
R-free | 0.26470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e9e |
RMSD bond length | 0.010 |
RMSD bond angle | 1.160 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 16435 | |
Completeness [%] | 96.3 | 97.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 20% PEG 4000, 0.1 M Nacitrate, 20% isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |