4E6I
Crystal structure of Staphylococcal nuclease variant NVIAGA V99G at cryogenic temperature
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Thermonuclease | polymer | 149 | 16738.3 | 2 | UniProt (P00644) Pfam (PF00565) In PDB | Staphylococcus aureus | TNase, Micrococcal nuclease, Staphylococcal nuclease |
| 2 | A | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 3 | B | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 1 | Chemie (MPD) | |||
| 4 | water | water | 18.0 | 94 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 149 (UniProt: P00644)
| PDB | External Database | Details |
|---|---|---|
| Asn 21 | Asp 103 | engineered mutation |
| Val 33 | Thr 115 | engineered mutation |
| Ile 41 | Thr 123 | engineered mutation |
| Ala 59 | Ser 141 | engineered mutation |
| Gly 99 | Val 181 | engineered mutation |
| Gly 117 | Pro 199 | engineered mutation |
| Ala 128 | Ser 210 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 33476.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 213.1 | |
| All* | Total formula weight | 33689.7 |
