4E6I

Crystal structure of Staphylococcal nuclease variant NVIAGA V99G at cryogenic temperature

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity
B	0003676	molecular_function	nucleic acid binding
B	0004518	molecular_function	nuclease activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PO4 A 201

Chain	Residue
A	ARG35
A	ARG87
A	HOH343
B	LYS71

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site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MPD B 201

Chain	Residue
A	LYS134
B	GLU10
B	TYR27
B	PHE76

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Functional Information from PROSITE/UniProt

site_id	PS01284
Number of Residues	11
Details	TNASE_2 Thermonuclease family signature 2. DKYGRgLAyIY

Chain	Residue	Details
A	ASP83-TYR93

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	32
Details	Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"evidences":[{"source":"PubMed","id":"288045","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	6
Details	Binding site: {}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 165

Chain	Residue	Details
A	ASN21	metal ligand
A	ARG35	electrostatic stabiliser, hydrogen bond donor
A	ASP40	metal ligand
A	ILE41	metal ligand
A	ARG87	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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site_id	MCSA2
Number of Residues	5
Details	M-CSA 165

Chain	Residue	Details
B	ASN21	metal ligand
B	ARG35	electrostatic stabiliser, hydrogen bond donor
B	ASP40	metal ligand
B	ILE41	metal ligand
B	ARG87	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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