4E4V

The crystal structure of the dimeric human importin alpha 1 at 2.5 angstrom resolution.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Importin subunit alpha-2	polymer	485	52665.5	2	UniProt (P52292) Pfam (PF01749) Pfam (PF00514) Pfam (PF16186)	Homo sapiens (human)	Karyopherin subunit alpha-2, RAG cohort protein 1, SRP1-alpha
2	C, D, E, F, G... (A, B)	GLYCEROL	non-polymer		92.1	13	Chemie (GOL)
3	K, Q (A, B)	2,3-DIHYDROXY-1,4-DITHIOBUTANE	non-polymer		154.3	2	Chemie (DTT)
4	R, S (A, B)	water	water		18.0	166	Chemie (HOH)

Sequence modifications

A, B: 70 - 529 (UniProt: P52292)

PDB	External Database	Details
Met 45	-	expression tag
Gly 46	-	expression tag
Ser 47	-	expression tag
Ser 48	-	expression tag
His 49	-	expression tag
His 50	-	expression tag
His 51	-	expression tag
His 52	-	expression tag
His 53	-	expression tag
Ser 54	-	expression tag
Ser 55	-	expression tag
Gly 56	-	expression tag
Glu 57	-	expression tag
Asn 58	-	expression tag
Leu 59	-	expression tag
Tyr 60	-	expression tag
Phe 61	-	expression tag
Gln 62	-	expression tag
Gly 63	-	expression tag
His 64	-	expression tag
Met 65	-	expression tag
Leu 66	-	expression tag
Asp 67	-	expression tag
Ala 68	-	expression tag
Leu 69	-	expression tag
Arg 486	Lys 486	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	105331.1
Non-Polymers*	Number of molecules	15
	Total formula weight	1505.7
All*	Total formula weight	106836.8

*Water molecules are not included.