4E0D
Binary complex of Bacillus DNA Polymerase I Large Fragment E658A and duplex DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase | polymer | 592 | 67416.3 | 1 | UniProt (Q5KWC1) Pfam (PF22619) Pfam (PF00476) | Geobacillus kaustophilus | |
| 2 | B (B) | DNA (5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(DOC))-3') | polymer | 9 | 2635.8 | 1 | Synthetic construct | ||
| 3 | C (C) | DNA (5'-D(*CP*AP*TP*GP*GP*GP*AP*GP*TP*CP*AP*GP*G)-3') | polymer | 13 | 4056.6 | 1 | Synthetic construct | ||
| 4 | D, E, F, G, H (A) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 5 | I, J, K (A) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 3 | Chemie (MPD) | |||
| 6 | L (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 7 | M (A) | 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE | non-polymer | 467.2 | 1 | Chemie (DCP) | |||
| 8 | N, O, P (A, B, C) | water | water | 18.0 | 824 | Chemie (HOH) |
Sequence modifications
A: 285 - 876 (UniProt: Q5KWC1)
| PDB | External Database | Details |
|---|---|---|
| Ala 598 | Asp 600 | engineered mutation |
| Ala 658 | Glu 660 | engineered mutation |
| His 823 | Arg 825 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 74108.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1497.2 | |
| All* | Total formula weight | 75605.9 |
